[Frontiers in Bioscience 11, 1108-1136, January 1, 2006] 1108 Mammalian genome targeting using site-specific recombinases
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Site-specific recombinases 3.1. Cre and FLP recombinases 3.2. Recombinases in a mammalian environment 3.3. Further developments 3.4. Novel recombinases 4. Technology to manipulate the mouse genome 4.1. Gene transfer 4.2. Gene targeting 4.3. Nuclear transfer 5. Strategies for conditional gene targeting 5.1. Generation of floxed alleles 5.2. Promoter-specific driven recombinase expression 5.3. Inducible systems 5.4. Virus-mediated delivery to specific cell or tissue types 5.5. Recombinase-activated siRNA expression 6. Approaches to model generation 6.1. Switching on/off genes in a spaceand time-controlled fashion 6.2. Chromosomal rearrangement 6.3. Cell lineage studies 6.4. Humanization of mouse genes 6.5. Cassette exchange or integration 7. Summary and perspectives 8. Acknowledgments 9. References
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